Code: 12316
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INTENDED USE
(For In Vitro Diagnostic Use Only)
Diaret-Prep.-CD 3.5 is a ready to use staining reagent for the staining of reticulocytes in whole blood sample for the determination of reticulocyte numbers on Cell-Dyn 3500/3700 hematology analysers.
SUMMARY AND PRINCIPLE
Determination of reticulocyte count in whole blood smear samples is performed routinely in hospital laboratories for the determination of erythropoetic activity in patients. Diaret-Prep.-CD 3.5 contains a nucleic acid staining thiazine dye. When the reagent is addded to a sample of whole blood the reticulocytic ribosomal RNA, the dye binds and simultaneously stains and precipitates the polyanion to form a network or reticulum. The stained reticulocytes are then determined in a Cell-Dyn 3500 flowcell by measuring the stained cells 10 and 90 degree light scatter intensities.